ABSTRACT
Abstract Oral rehabilitation with osseointegrated implants is a way to restore esthetics and masticatory function in edentulous patients, but bacterial colonization around the implants may lead to mucositis or peri-implantitis and consequent implant loss. Peri-implantitis is the main complication of oral rehabilitation with dental implants and, therefore, it is necessary to take into account the potential effects of antiseptics such as chlorhexidine (CHX), chloramine T (CHT), triclosan (TRI), and essential oils (EO) on bacterial adhesion and on biofilm formation. To assess the action of these substances, we used the microcosm technique, in which the oral environment and periodontal conditions are simulated in vitro on titanium discs with different surface treatments (smooth surface - SS, acid-etched smooth surface - AESS, sand-blasted surface - SBS, and sand-blasted and acid-etched surface - SBAES). Roughness measurements yielded the following results: SS: 0.47 µm, AESS: 0.43 µm, SB: 0.79 µm, and SBAES: 0.72 µm. There was statistical difference only between SBS and AESS. There was no statistical difference among antiseptic treatments. However, EO and CHT showed lower bacterial counts compared with the saline solution treatment (control group). Thus, the current gold standard (CHX) did not outperform CHT and EO, which were efficient in reducing the biofilm biomass compared with saline solution.
Subject(s)
Humans , Titanium/chemistry , Bacterial Adhesion/drug effects , Biofilms/drug effects , Anti-Infective Agents, Local/pharmacology , Mouthwashes/pharmacology , Saliva/microbiology , Surface Properties/drug effects , Time Factors , Tosyl Compounds/pharmacology , Tosyl Compounds/chemistry , Triclosan/pharmacology , Triclosan/chemistry , Oils, Volatile/pharmacology , Oils, Volatile/chemistry , Chloramines/pharmacology , Chloramines/chemistry , Chlorhexidine/pharmacology , Chlorhexidine/chemistry , Reproducibility of Results , Analysis of Variance , Biofilms/growth & development , Bacterial Load , Anti-Infective Agents, Local/chemistry , Mouthwashes/chemistryABSTRACT
Valores Feulgen-DNA e áreas absorventes nucleares foram determinados por citofotometria de varredura em esfregaços de espermatozóides de touro tratados com uma soluçäo a 2% de cloramina-T (agente utilizado para remoçäo do muco presente no sêmen) por 5 a 15 min. Pôde ser demonstrado que o tratamento por cloramina-T mesmo por curtos períodos de tempo näo é recomendável para preparados a serem usados na detecçäo de conteúdos de DNA, pois o mesmo remove proteínas nucleares, afetando a cinética de hidrólise de Feulgen da cromatina de modo näo homogêneo